The built environment impacts health in multiple dimensions, from large infrastructure to the microscopic molecules and organisms that are not seen or perceived in daily life. In light of the complex interaction between various health-related impacts of the built environment, this publication has taken a multi-disciplinary approach using the Bullitt Center as a pilot project to develop and implement methodologies for collecting data. These data include testing how the building impacts 1) physical activity and 2) indoor environmental quality, including the microbiome. This paper outlines the relevant background information, active design and indoor environmental quality studies, and shares preliminary findings.